771 skills found · Page 1 of 26
jdepoix / Youtube Transcript ApiThis is a python API which allows you to get the transcript/subtitles for a given YouTube video. It also works for automatically generated subtitles and it does not require an API key nor a headless browser, like other selenium based solutions do!
folke / Which Key.nvim💥 Create key bindings that stick. WhichKey helps you remember your Neovim keymaps, by showing available keybindings in a popup as you type.
streaak / KeyhacksKeyhacks is a repository which shows quick ways in which API keys leaked by a bug bounty program can be checked to see if they're valid.
microsoft / LLMLingua[EMNLP'23, ACL'24] To speed up LLMs' inference and enhance LLM's perceive of key information, compress the prompt and KV-Cache, which achieves up to 20x compression with minimal performance loss.
NAalytics / Assemblies Of Putative SARS CoV2 Spike Encoding MRNA Sequences For Vaccines BNT 162b2 And MRNA 1273RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
aserbao / AndroidCamera🔥🔥🔥自定义Android相机(仿抖音 TikTok)，其中功能包括视频人脸识别贴纸，美颜，分段录制，视频裁剪，视频帧处理，获取视频关键帧，视频旋转，添加滤镜，添加水印，合成Gif到视频，文字转视频，图片转视频，音视频合成，音频变声处理，SoundTouch，Fmod音频处理。 Android camera(imitation Tik Tok), which includes video editor,audio editor,video face recognition stickers, segment recording,video cropping, video frame processing, get the first video frame, key frame, video rotation, add filter Mirror ,add watermark ,add gif to video, add text to video, picture to video, audio and video synthesis, audio change processing, SoundTouch, Fmod audio processing.
dmauro / KeypressA keyboard input capturing utility in which any key can be a modifier key.
liuchengxu / Vim Which Key:tulip: Vim plugin that shows keybindings in popup
justbur / Emacs Which KeyEmacs package that displays available keybindings in popup
mrjones2014 / Legendary.nvim🗺️ A legend for your keymaps, commands, and autocmds, integrates with which-key.nvim, lazy.nvim, and more.
kimamula / Ts Transformer KeysA TypeScript custom transformer which enables to obtain keys of given type
mtahiraslan / Data Analyst RoadmapBased on my own experience, I believe this roadmap provides clear answers to all the key questions about becoming a data analyst from scratch, including which technologies and programming languages to learn, what soft skills are essential, and how to start a professional career in this field.
resque / Redis NamespaceThis gem adds a Redis::Namespace class which can be used to namespace Redis keys.
wk8 / Go Ordered MapOptimal implementation of ordered maps for Golang - ie maps that remember the order in which keys were inserted.
rehooks / Local StorageReact hook which syncs localStorage[key] with the comp.
Tencent / FanvasFanvas，一键把swf转为html5 canvas动画。 Fanvas is a tool which can turn flash into canvas animation with just one key!
dogtagpki / PkiThe Dogtag Certificate System is an enterprise-class Certificate Authority (CA) which supports all aspects of certificate lifecycle management, including key archival, OCSP and smartcard management.
iMasanari / Cmd EikanaApplication of macOS which switches Alphabet / Kana by pressing left and right command key alone. Other key remapping is also possible.
b0o / Surfingkeys Conf🏄 A SurfingKeys config which adds 180+ key mappings & 50+ search engines
PushpenderIndia / SinisterSinister is Windows/Linux Keylogger Generator which sends key-logs via email with other juicy target info
