2,857 skills found · Page 1 of 96
winapps-org / WinappsRun Windows apps such as Microsoft Office/Adobe in Linux (Ubuntu/Fedora) and GNOME/KDE as if they were a part of the native OS, including Nautilus integration. Hard fork of https://github.com/Fmstrat/winapps/
wojtekmaj / React PdfDisplay PDFs in your React app as easily as if they were images.
Fmstrat / WinappsRun Windows apps such as Microsoft Office/Adobe in Linux (Ubuntu/Fedora) and GNOME/KDE as if they were a part of the native OS, including Nautilus integration.
norman / Friendly IdFriendlyId is the “Swiss Army bulldozer” of slugging and permalink plugins for ActiveRecord. It allows you to create pretty URL’s and work with human-friendly strings as if they were numeric ids for ActiveRecord models.
NAalytics / Assemblies Of Putative SARS CoV2 Spike Encoding MRNA Sequences For Vaccines BNT 162b2 And MRNA 1273RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
Esqarrouth / EZSwiftExtensions:smirk: How Swift standard types and classes were supposed to work.
obi1kenobi / TrustfallA query engine for any combination of data sources. Query your files and APIs as if they were databases!
SHUR1K-N / Flipper Zero Sub GHz Jamming FilesLooking for those jamming files that were removed from custom firmwares? Here they are. Only for educational purposes, of course.
jOOQ / JOOLjOOλ - The Missing Parts in Java 8 jOOλ improves the JDK libraries in areas where the Expert Group's focus was elsewhere. It adds tuple support, function support, and a lot of additional functionality around sequential Streams. The JDK 8's main efforts (default methods, lambdas, and the Stream API) were focused around maintaining backwards compatibility and implementing a functional API for parallelism.
spacemeowx2 / Switch Lan PlayEnjoy games with your friends as if you were on a LAN.
git-commit-id / Git Commit Id Maven PluginMaven plugin which includes build-time git repository information into an POJO / *.properties). Make your apps tell you which version exactly they were built from! Priceless in large distributed deployments... :-)
Bloomberg-Beta / ManualYou were probably looking for our website... this is it. We moved our website here, so you can see the insides of how we work.
sdras / Vue Vscode SnippetsThese snippets were built to supercharge my workflow in the most seamless manner possible.
Nigh / I Wanna Clean KeyboardBlock the keyboard input while you were eating instant noodles on your laptop keyboard.
o0o0o0o0o0o0o / Image Processing From ScratchThis project contains some interesting image processing algorithms that were wrote in python and c++ from scratch.
msadowski / Awesome Weekly RoboticsA list of projects that were or will be featured in Weekly Robotics newsletter
atiilla / GeoIntelGeoIntel using Google's Gemini API to uncover the location where photos were taken through AI-powered geo-location analysis.
grodowski / Undercoverundercover warns about methods, classes and blocks that were changed without tests, to help you easily find untested code and reduce the number of bugs. It does so by analysing data from git diffs, code structure and SimpleCov coverage reports
wangjs96 / A Tutorial Compiler Written In JavaFor Jack language. Most of codes were commented with their usage, which can be useful for beginner to realize the running principle of a compiler for object-oriented programming language.
enormego / EGOImageLoadingWhat if images on the iPhone were as easy as HTML?
