1,188 skills found · Page 1 of 40
QianMo / Game Programmer Study Notes:anchor: 我的游戏程序员生涯的读书笔记合辑。你可以把它看作一个加强版的Blog。涉及图形学、实时渲染、编程实践、GPU编程、设计模式、软件工程等内容。Keep Reading , Keep Writing , Keep Coding.
keyvanakbary / Learning NotesNotes on books I read, talks I watch, articles I study, and papers I love
LyleMi / Learn Web HackingStudy Notes For Web Hacking / Web安全学习笔记
CharonChui / AndroidNoteAndroid study notes.
kangjianwei / Data Structure《数据结构》-严蔚敏.吴伟民-教材源码与习题解析
NAalytics / Assemblies Of Putative SARS CoV2 Spike Encoding MRNA Sequences For Vaccines BNT 162b2 And MRNA 1273RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
QianMo / Real Time Rendering 3rd CN Summary Ebook:blue_book: 电子书 -《Real-Time Rendering 3rd》提炼总结 | 全书共9万7千余字。你可以把它看做中文通俗版的《Real-Time Rendering 3rd》，也可以把它看做《Real-Time Rendering 3rd》的解读版与配套学习伴侣，或者《Real-Time Rendering 4th》的前置阅读材料。
hesamsheikh / Ml RetreatMachine Learning Journal for Intermediate to Advanced Topics.
BlackZhangJX / Android NotesAndroid开发核心知识点笔记（不断更新中🔥）
HarleyCoops / Math To ManimCreate Epic Math and Physics Animations & Study Notes From Text and Images.
skulltech / Aws Solutions Architect Associate NotesMy notes for AWS Solutions Architect Associate.
neomatrix369 / Awesome AI Ml DlAwesome Artificial Intelligence, Machine Learning and Deep Learning as we learn it. Study notes and a curated list of awesome resources of such topics.
kangjianwei / LearningJDKJDK源码阅读笔记
CaviraOSS / PageLMPageLM is a community driven version of NotebookLM & a education platform that transforms study materials into interactive resources like quizzes, flashcards, notes, and podcasts.
CNFeffery / DataScienceStudyNotes这个仓库保管从（数据科学学习手札69）开始的所有代码、数据等相关附件内容
tsrot / Study NotesWEB前端学习笔记和感悟。If you don’t learn to think when you are young, you may never learn.
djytmdj / Network Security Study Notes主要记录网络安全学习笔记，包含WEB安全、提权、APP渗透、内网渗透、横向移动、红队、工具学习等
krislinzhao / StudyNotes📚JAVA、Spring、SpringMVC、SpringBoot、Mybatis、Vue、MySQL、MongoDB、Radis、Docker、Nginx、......笔记
codinglin / StudyNotes个人学习笔记
L1aoXingyu / Roadmap Of DL And MLRoadmap of DL and ML, some courses, study notes and paper summary
