2,445 skills found · Page 1 of 82
microsoft / MS DOSThe original sources of MS-DOS 1.25, 2.0, and 4.0 for reference purposes
subframe7536 / Maple FontMaple Mono: Open source monospace font with round corner, ligatures and Nerd-Font icons for IDE and terminal, fine-grained customization options. 带连字和控制台图标的圆角等宽字体，中英文宽度完美2:1，细粒度的自定义选项
rustfs / Rustfs🚀2.3x faster than MinIO for 4KB object payloads. RustFS is an open-source, S3-compatible high-performance object storage system supporting migration and coexistence with other S3-compatible platforms such as MinIO and Ceph.
netbox-community / NetboxThe premier source of truth powering network automation. Open source under Apache 2. Try NetBox Cloud free: https://netboxlabs.com/products/free-netbox-cloud/
cocos2d / Cocos2d XCocos2d-x is a suite of open-source, cross-platform, game-development tools utilized by millions of developers across the globe. Its core has evolved to serve as the foundation for Cocos Creator 1.x & 2.x.
OpenRCT2 / OpenRCT2An open source re-implementation of RollerCoaster Tycoon 2 🎢
google-deepmind / AlphafoldOpen source code for AlphaFold 2.
dragen1860 / Deep Learning With TensorFlow Book深度学习入门开源书，基于TensorFlow 2.0案例实战。Open source Deep Learning book, based on TensorFlow 2.0 framework.
beemdevelopment / AegisA free, secure and open source app for Android to manage your 2-step verification tokens.
OpenDiablo2 / OpenDiablo2An open source re-implementation of Diablo 2
Activiti / ActivitiActiviti is a light-weight workflow and Business Process Management (BPM) Platform targeted at business people, developers and system admins. Its core is a super-fast and rock-solid BPMN 2 process engine for Java. It's open-source and distributed under the Apache license. Activiti runs in any Java application, on a server, on a cluster or in the cloud. It integrates perfectly with Spring, it is extremely lightweight and based on simple concepts.
TrinityCore / TrinityCoreTrinityCore Open Source MMO Framework (master = 12.0.1.66838, 3.3.5 = 3.3.5a.12340, cata classic = 4.4.2.60895)
microsoft / WSL2 Linux KernelThe source for the Linux kernel used in Windows Subsystem for Linux 2 (WSL2)
ValveSoftware / Source SDK 2013The 2013 edition of the Source SDK
GreptimeTeam / GreptimedbThe open-source Observability 2.0 database. One engine for metrics, logs, and traces — replacing Prometheus, Loki & ES.
Facepunch / Sbox Publics&box is a modern game engine, built on Valve's Source 2 and the latest .NET technology, it provides a modern intuitive editor for creating games
baserow / BaserowBuild databases, automations, apps & agents with AI — no code. Open source platform available on cloud and self-hosted. GDPR, HIPAA, SOC 2 compliant. Best Airtable alternative.
nkaz001 / HftbacktestFree, open source, a high frequency trading and market making backtesting and trading bot, which accounts for limit orders, queue positions, and latencies, utilizing full tick data for trades and order books(Level-2 and Level-3), with real-world crypto trading examples for Binance and Bybit
NAalytics / Assemblies Of Putative SARS CoV2 Spike Encoding MRNA Sequences For Vaccines BNT 162b2 And MRNA 1273RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
id-Software / Quake 2Quake 2 GPL Source Release
