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streamlit / StreamlitStreamlit — A faster way to build and share data apps.
eugeneyan / Applied Ml📚 Papers & tech blogs by companies sharing their work on data science & machine learning in production.
getredash / RedashMake Your Company Data Driven. Connect to any data source, easily visualize, dashboard and share your data.
yjs / YjsShared data types for building collaborative software
fossasia / VisdomA flexible tool for creating, organizing, and sharing visualizations of live, rich data. Supports Torch and Numpy
drivendataorg / Cookiecutter Data ScienceA logical, reasonably standardized, but flexible project structure for doing and sharing data science work.
jtleek / DatasharingThe Leek group guide to data sharing
airbnb / Knowledge RepoA next-generation curated knowledge sharing platform for data scientists and other technical professions.
ckan / CkanCKAN is an open-source DMS (data management system) for powering data hubs and data portals. CKAN makes it easy to publish, share and use data. It powers catalog.data.gov, open.canada.ca/data, data.humdata.org among many other sites.
windiest / Front End Tutorial:smiley_cat:猫的前端回忆录 Cat's front memory, these share data are from my usual work and learning, hoping to help you, and hoping slowly improve, if you like you can star
StructuredLabs / PreswaldPreswald is a WASM packager for Python-based interactive data apps: bundle full complex data workflows, particularly visualizations, into single files, runnable completely in-browser, using Pyodide, DuckDB, Pandas, and Plotly, Matplotlib, etc. Build dashboards, reports, and notebooks that run offline, load fast, and share like a document.
react-native-share / React Native ShareSocial share, sending simple data to other apps.
NAalytics / Assemblies Of Putative SARS CoV2 Spike Encoding MRNA Sequences For Vaccines BNT 162b2 And MRNA 1273RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
terminusdb / TerminusdbTerminusDB is a distributed, collaborative database designed for building, sharing, versioning, and reasoning on structured data.
dotnet / Interactive.NET Interactive combines the power of .NET with many other languages to create notebooks, REPLs, and embedded coding experiences. Share code, explore data, write, and learn across your apps in ways you couldn't before.
charted-co / ChartedA charting tool that produces automatic, shareable charts from any data file
mapbox / Geojson.ioA quick, simple tool for creating, viewing, and sharing spatial data
coresmart / Persistencejspersistence.js is an asynchronous Javascript database mapper library. You can use it in the browser, as well on the server (and you can share data models between them).
Peer5 / ShareFestWeb based p2p file sharing built on WebRTC Data Channels API
GeoNode / GeonodeGeoNode is an open source platform that facilitates the creation, sharing, and collaborative use of geospatial data.
