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affaan-m / Everything Claude CodeThe agent harness performance optimization system. Skills, instincts, memory, security, and research-first development for Claude Code, Codex, Opencode, Cursor and beyond.
The-Art-of-Hacking / H4ckerThis repository is maintained by Omar Santos (@santosomar) and includes thousands of resources related to ethical hacking, bug bounties, digital forensics and incident response (DFIR), AI security, vulnerability research, exploit development, reverse engineering, and more. 🔥 Also check: https://hackertraining.org
microsoft / RD AgentResearch and development (R&D) is crucial for the enhancement of industrial productivity, especially in the AI era, where the core aspects of R&D are mainly focused on data and models. We are committed to automating these high-value generic R&D processes through R&D-Agent, which lets AI drive data-driven AI. 🔗https://aka.ms/RD-Agent-Tech-Report
open-mmlab / AmphionAmphion (/æmˈfaɪən/) is a toolkit for Audio, Music, and Speech Generation. Its purpose is to support reproducible research and help junior researchers and engineers get started in the field of audio, music, and speech generation research and development.
PetoiCamp / OpenCat Quadruped RobotAn open source quadruped robot pet framework for developing Boston Dynamics-style four-legged robots that are perfect for STEM, coding & robotics education, IoT robotics applications, AI-enhanced robotics application services, research, and DIY robotics kit development.
NAalytics / Assemblies Of Putative SARS CoV2 Spike Encoding MRNA Sequences For Vaccines BNT 162b2 And MRNA 1273RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
Galaxy-Dawn / Claude ScholarSemi-automated research assistant for academic research and software development. Supports Claude Code, OpenCode, and Codex CLI across ideation, coding, experiments, writing, and publication.
mzz2017 / Gg一个支持节点与订阅链接的 Linux 命令行代理工具 | A command-line tool for one-click proxy in your research and development without installing v2ray or anything else (only for linux)
tgfrerer / Island🌋🐎 Project Island is an experimental, hot-reloading Vulkan Renderer for Linux and Windows, written in C/C++.
NPC-Worldwide / NpcpyThe python library for research and development in NLP, multimodal LLMs, Agents, ML, Knowledge Graphs, and more.
InternRobotics / InternUtopiaA simulation platform for versatile Embodied AI research and developments.
pthom / Imgui BundleInteractive Python & C++ apps for desktop, mobile, and web - powered by Dear ImGui. Stop fighting GUI frameworks. Start building.
PromtEngineer / VerbiA modular voice assistant application for experimenting with state-of-the-art transcription, response generation, and text-to-speech models. Supports OpenAI, Groq, Elevanlabs, CartesiaAI, and Deepgram APIs, plus local models via Ollama. Ideal for research and development in voice technology.
DmitryRyumin / ICCV 2023 25 PapersICCV 2023-2025 Papers: Discover cutting-edge research from ICCV 2023-25, the leading computer vision conference. Stay updated on the latest in computer vision and deep learning, with code included. ⭐ support visual intelligence development!
precice / PreciceA coupling library and ecosystem for partitioned multi-physics and multi-scale simulations, including surface and volume coupling.
grumpycoders / Pcsx ReduxThe PCSX-Redux project is a collection of tools, research, hardware design, and libraries aiming at development and reverse engineering on the PlayStation 1. The core product itself, PCSX-Redux, is yet another fork of the Playstation emulator, PCSX.
marin-community / MarinOpen-source framework for the research and development of foundation models.
ronin-rb / RoninRonin is a Free and Open Source Ruby Toolkit for Security Research and Development. Ronin also allows for the rapid development and distribution of code, exploits, payloads, etc, via 3rd-party git repositories.
primeqa / PrimeqaThe prime repository for state-of-the-art Multilingual Question Answering research and development.
gchq / BoilingFrogsGCHQ's internal Boiling Frogs research paper on software development and organisational change in the face of disruption #boilingfrogs
