1,481 skills found · Page 1 of 50
PHP-CS-Fixer / PHP CS FixerA tool to automatically fix PHP Coding Standards issues
squizlabs / PHP CodeSnifferPHP_CodeSniffer tokenizes PHP files and detects violations of a defined set of coding standards.
Kristories / Awesome GuidelinesA curated list of high quality coding style conventions and standards.
codeguy / Php The Right WayAn easy-to-read, quick reference for PHP best practices, accepted coding standards, and links to authoritative tutorials around the Web
checkstyle / CheckstyleCheckstyle is a development tool to help programmers write Java code that adheres to a coding standard. By default it supports the Google Java Style Guide and Sun Code Conventions, but is highly configurable. It can be invoked with an ANT task and a command line program.
jstherightway / Js The Right WayAn easy-to-read, quick reference for JS best practices, accepted coding standards, and links around the Web
vipshop / VjtoolsThe vip.com's java coding standard, libraries and tools
mukul975 / Anthropic Cybersecurity Skills753+ structured cybersecurity skills for AI agents · MITRE ATT&CK mapped · agentskills.io open standard · Works with Claude Code, GitHub Copilot, OpenAI Codex CLI, Cursor, Gemini CLI & 20+ platforms · Penetration testing, DFIR, threat intel, cloud security & more · Apache 2.0
NAalytics / Assemblies Of Putative SARS CoV2 Spike Encoding MRNA Sequences For Vaccines BNT 162b2 And MRNA 1273RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
carthage-software / MagoMago is a toolchain for PHP that aims to provide a set of tools to help developers write better code.
WordPress / WordPress Coding StandardsPHP_CodeSniffer rules (sniffs) to enforce WordPress coding conventions
EONRaider / Blackhat Python3Source code for the book "Black Hat Python" by Justin Seitz. The code has been fully converted to Python 3, reformatted to comply with PEP8 standards and refactored to eliminate dependency issues involving the implementation of deprecated libraries.
transmissions11 / SolcurityOpinionated security and code quality standard for Solidity smart contracts.
easy-coding-standard / Easy Coding StandardThe Easiest way to add coding standard to your PHP project
stefangabos / World CountriesConstantly updated lists of world countries and their associated alpha-2, alpha-3 and numeric country codes as defined by the ISO 3166 standard, available in CSV, JSON , PHP, SQL and XML formats, in multiple languages and with national flags included; also available are the ISO 3166-2 codes of provinces/ states associated with the countries
SalGnt / CscsA curated list of Coding Style Conventions and Standards.
Warrenren / Inside Rust Std Library本书已经正式出版，目前正预售，可在京东搜索《深入RUST标准库》即可。本书主要对RUST的标准库代码进行分析，并试图给出RUST标准库代码的分析脉络。This project try to give a venation of how reading the RUST standard library source code.
PHPCSStandards / PHP CodeSnifferPHP_CodeSniffer tokenizes PHP files and detects violations of a defined set of coding standards.
slevomat / Coding StandardSlevomat Coding Standard for PHP_CodeSniffer provides many useful sniffs
EthicalML / Awesome Artificial Intelligence RegulationThis repository aims to map the ecosystem of artificial intelligence guidelines, principles, codes of ethics, standards, regulation and beyond.
