EnzymeDesign

Enzyme search using ESM-1b and colabfold database This colab enables you to find potential proteins that will act similarly as your input enzymes. You will need to provide a fasta file with any numerical value representing your enzymes (i.e. Km, Kcat/Km, toxicity, ...), then the colab will give you a list of proteins similar to the given enzymes, sorted by the numerical value predicted by the regressor.

<br> <b>What you need to prepare</b>

• A fasta file containing the sequence and numerical value representing the sequence (see the example fasta format below)

<b>Example fasta format</b>:

>Example_sequence_1 8.104<br> PIAQIHILEGRSDEQKETLIREVSEAISRSLDAPLTSVRVIITEMAKGHFGIGGELASK<br> >Example_sequence_2 2.04<br> MIAQIHILEGRSDKETLIRRVSEAISRSLDAPLTSVRVIIMEMAKGHFGIGGELASK<br> >Example_sequence_3 5.20<br> SIATIHILEGRSDEDKETLIRRVISRSLDAPLTSVRVIIMTMAKKHFGIGGELASK