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** Fast5 Library

[[http://travis-ci.org/mateidavid/fast5][http://travis-ci.org/mateidavid/fast5.svg?branch=master]] [[https://tldrlegal.com/license/mit-license][http://img.shields.io/:license-mit-blue.svg]]

A lightweight C++ library for accessing Oxford Nanopore Technologies sequencing data.

*** Installation

**** Core C++ Library

The core library is written in header-only C++11, and it enables read-write access to fast5 files from C++ code.

The core library requires no installation, other than setting the compiler's include path to find [[file:include/fast5.hpp][fast5.hpp]]. See [[file:src/f5ls-full.cpp][f5ls-full.cpp]] for an example.

The core library is built on top the HDF5 C API, so the compiler must also be able to find the HDF5 headers and libraries. See the project's Travis CI [[file:.travis.Dockerfile.in][Dockerfile]] for an example of how to install prerequisites on Debian Jessie.

**** Python Wrapper

The Python wrapper for the core library enables read-only access to fast5 files from Python code. The wrapper also adds several Python scripts:

• [[file:python/bin/f5ls][f5ls]] :: Summarize contents of fast5 files.
• [[file:python/bin/f5pack][f5pack]] :: Pack and unpack fast5 files. For a detailed description of this tool, see our [[http://simpsonlab.github.io/2017/02/27/packing_fast5/][blog post]].

The Python wrapper also depends on Cython. To build the Python wrapper:

#+BEGIN_EXAMPLE

set paths to HDF5

export HDF5_INCLUDE_DIR=/path/to/hdf5.h export HDF5_LIB_DIR=/path/to/libhdf5.so

either

make -C python develop # to install in a virtualenv

or

make -C python develop-user # to install in user mode #+END_EXAMPLE

*** License

[[file:LICENSE][MIT License]].