brainGraph

brainGraph (RRID: SCR_017260) is an R package for performing graph theory analyses of brain MRI data. It is most useful in atlas-based analyses (e.g., using an atlas such as AAL, or one from Freesurfer); however, many of the computations (e.g., the GLM-based functions and the network-based statistic) will work with any graph that is compatible with igraph. The package will perform analyses for structural covariance networks (SCN), DTI tractography (I use probtrackx2 from FSL), and resting-state fMRI covariance (I have used the Matlab-based DPABI toolbox).

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• Requirements
  • Operating Systems
  • Multi-core processing
• Compatibility
  • Neuroimaging software
  • Brain atlases
    • Other atlases
• Installation
  • Multi-core processing
  • GUI
  • Suggested packages
• Usage - the User Guide
• Major changes in v3.0.0
• Graph measures
  • Group analyses
    • GLM-based
    • Non-GLM based
  • Null graph-related measures
  • Other measures
• Visualization
• Getting Help
• Future versions

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Requirements

Operating Systems

The package should work "out-of-the-box" on Linux systems (at least on Red Hat-based systems; i.e., CentOS, RHEL, Scientific Linux, etc.) since almost all development (and use, by me) has been on computers running CentOS 6 and (currently) CentOS 7. I have also had success running it (and did some development) on Windows 7, and have heard from users that it works on some versions of Mac OS and on Ubuntu. Please see the User Guide (mentioned below) for more details.

Multi-core processing

Many brainGraph functions utilize multiple CPU cores. This is primarily done via the foreach package. Depending on your OS, you will need to install doMC (macOS and Linux) or doSNOW (Windows).

Compatibility

Neuroimaging software

I mostly use Freesurfer and FSL, but the following software packages should be suitable. Note that this is an incomplete list; any software that can output a connectivity matrix will work.

• Freesurfer
• FSL
• DPARSF
• PANDA
• TrackVis

Brain atlases

There are several brain atlases for which the data are present in brainGraph. Atlases containing .scgm in the name contain both cortical and SubCortical Gray Matter (SCGM) regions.

1. dk and dk.scgm: Desikan-Killiany
2. dkt and dkt.scgm: Desikan-Killiany-Tourville
3. destrieux and destrieux.scgm: Destrieux
4. aal90 and aal116: Automated Anatomical Labeling atlas
5. aal2.94 and aal2.120: AAL-2
6. brainsuite: Brainsuite
7. craddock200: Craddock-200
8. dosenbach160: Dosenbach-160
9. hoa112: Harvard-Oxford atlas
10. lpba40: LONI Probabilistic Brain Atlas
11. hcp_mmp1.0: HCP-1mm
12. power264: Power-264
13. gordon333: Gordon-333
14. brainnetome: Brainnetome

Other atlases

Some functions accept a custom.atlas argument, so that you can analyze data that is from an atlas not present in brainGraph. Other atlases to be added in the future include the following (I would need specific coordinate, region name, and lobe and hemisphere information):

• Shen-268
• Von Economo-Koskinas
• Willard-499 (see Richiardi et al., 2015)
• Schaefer-400 (see Schaefer et al., 2018)

Installation

There are (primarily) two ways to install this package:

1. Directly from CRAN: (use one of the following commands)

install.packages('brainGraph')
install.packages('brainGraph', dependencies=TRUE)

2. From the GitHub repo (for development versions). This requires that the devtools package be installed:

devtools::install_github('cwatson/brainGraph')

This should install all of the dependencies needed along with the package itself. For more details, see the User Guide (PDF link).

Multi-core processing

To set up your R session for parallel processing, you can use the following code. Note that it is different for Windows. This code should be run before any data processing. If you will always use a single OS, you can remove the unnecessary lines.

OS <- .Platform$OS.type
if (OS == 'windows') {
  library(snow)
  library(doSNOW)
  num.cores <- as.numeric(Sys.getenv('NUMBER_OF_PROCESSORS'))
  cl <- makeCluster(num.cores, type='SOCK')
  clusterExport(cl, 'sim.rand.graph.par')   # Or whatever functions you will use
  registerDoSNOW(cl)
} else {
  library(doMC)
  registerDoMC(detectCores() - 1L)  # Keep 1 core free
}

For example, I source the following simple script before I do any parallel processing with brainGraph:

pacman::p_load(brainGraph, doMC)
registerDoMC(detectCores())

GUI

On some systems (e.g., macOS and Windows) it might be difficult to install the necessary packages/dependencies for the GUI functions. Since v2.2.0 (released 2018-05-28), the R packages RGtk2 and cairoDevice have been changed to Suggests (i.e., they are no longer required), so it can be installed on a "headless" server.

If you are on macOS or Windows and would like GUI functionality, please see this GitHub Gist. The comments contain more recent information. You may also need to install a few additional packages, shown here:

install.packages('gWidgets', dependencies=TRUE)
install.packages('gWidgetsRGtk2', dependencies=TRUE)
install.packages('RGtk2Extras', dependencies=TRUE)

Suggested packages

There are a few suggested packages that may be required for certain functions:

• RGtk2 and cairoDevice: as mentioned above, these are required to use the GUI
• boot: required for brainGraph_boot
• Hmisc: required for corr.matrix
• ade4: required for loo and aop
• expm: required for communicability and centr_betw_comm

Usage - the User Guide

I have a User Guide that contains extensive code examples for analyses common to brain MRI studies. I also include some code for getting your data into R from Freesurfer, FSL, and DPABI, and some suggestions for workflow organization.

The User Guide is the most complete documentation of this package. If you are a beginner using R, I encourage you to read it thoroughly. You may start with the Preface or at whichever chapter is suitable for your analyses.

Major changes in v3.0.0

There are several major changes in v3.0.0. See the User Guide for more extensive details.

• There are several fewer package dependencies, allowing for a quicker install process
• There are a few new built-in atlases (see below for the full list)
• Graph creation is si