Caravagene

.. raw:: html

<p align="center">
<img alt="Caravagene logo" title="Caravagene logo" src="https://raw.githubusercontent.com/Edinburgh-Genome-Foundry/Caravagene/master/logo.png" width="500">
<br /><br />
</p>

.. image:: https://github.com/Edinburgh-Genome-Foundry/Caravagene/actions/workflows/build.yml/badge.svg :target: https://github.com/Edinburgh-Genome-Foundry/Caravagene/actions/workflows/build.yml :alt: GitHub CI build status

.. image:: https://coveralls.io/repos/github/Edinburgh-Genome-Foundry/Caravagene/badge.svg?branch=master :target: https://coveralls.io/github/Edinburgh-Genome-Foundry/Caravagene?branch=master

Caravagene (full documentation here <https://edinburgh-genome-foundry.github.io/Caravagene/>_) is a Python library to plot schemas of DNA constructs from a list of parts:

.. code:: python

from caravagene import Part, Construct, ConstructList

constructs = ConstructList([Construct([
    Part('promoter', label='my promoter'),
    Part('CDS', label='gene with a very very long name'),
    Part('terminator', label='PolyA'),
    Part('insulator', label='I1')
])])

constructs.to_image('construct.jpeg')

.. raw:: html

<p align="center">
<img src="https://raw.githubusercontent.com/Edinburgh-Genome-Foundry/Caravagene/master/examples/construct.jpeg" width="600">
</p>

Here is another example producing this PDF <https://raw.githubusercontent.com/Edinburgh-Genome-Foundry/Caravagene/master/examples/multiconstruct.pdf>_ showing multiple constructs:

.. code:: python

from caravagene import Part, Construct, ConstructList

my_constructs = ConstructList(
    title="My constructs",
    constructs=[
        Construct(name="ASM1", parts=[
            Part(label='HA1', category='homology-arm'),
            Part(label='rc1', category='recombinase-recognition-sequence'),
            Part(label='my promoter', category='promoter'),
            Part(label='RNA stability', category='rna-stability-sequence'),
            Part(label='<i>acs</i>', category='CDS'),
            Part(label='PolyA', category='terminator'),
            Part(label='I1', category='insulator'),
        ]),
        Construct(name="ASM2", parts=[
            Part(label='my promoter', category='promoter'),
            Part(label='gene with a very very long name', category='CDS'),
            Part(label='PolyA', category='terminator'),
            Part(label='I1', category='insulator')
        ])
    ]
)
my_constructs.to_pdf('multiconstruct.pdf')

.. raw:: html

<p align="center">
<img src="https://raw.githubusercontent.com/Edinburgh-Genome-Foundry/Caravagene/master/examples/multiconstruct_screen_capture.png" width="800">
</p>

Note that ConstructList can be supplied with a font. It is also possible to extend Caravagene to support other categories/symbols, as follows:

.. code:: python

 from caravagene import SYMBOL_FILES
 SYMBOL_FILES['my-new-category'] = 'path/to/some/symbol.svg'

Finally, here is an example using an Excel spreadsheet:

Spreadsheet:

.. raw:: html

<p align="center">
<img src="https://raw.githubusercontent.com/Edinburgh-Genome-Foundry/Caravagene/master/examples/from_spreadsheet_sample.png" width="600">
</p>

Python code:

.. code:: python

from caravagene import ConstructList
my_constructs = ConstructList("my_spreadsheet.xlsx")
my_constructs.to_pdf('my_schemas.pdf')

or command-line (one-time use):

.. code:: shell

caravagene my_spreadsheet.xlsx my_schemas.pdf

or command-line (re-render when the spreadsheet changes on disk):

.. code:: shell

caravagene my_spreadsheet.xlsx my_schemas.pdf --watch

Output:

.. raw:: html

<p align="center">
<img src="https://raw.githubusercontent.com/Edinburgh-Genome-Foundry/Caravagene/master/examples/from_spreadsheet_screen_capture.png" width="700">
</p>

Installation

Caravagene requires WkHTMLtoPDF <https://wkhtmltopdf.org/>_ to be installed. On Ubuntu, install it with:

.. code:: shell

apt-get install wkhtmltopdf

You can install caravagene through PIP:

.. code:: shell

pip install caravagene

For using it in the terminal, install with:

.. code:: shell

pip install caravagene[cli]

Licence

Caravagene is an open-source software originally written at the Edinburgh Genome Foundry <http://www.genomefoundry.org>_ by Zulko <https://github.com/Zulko>_ and released on Github <https://github.com/Edinburgh-Genome-Foundry/Caravagene>_ under the MIT licence (Copyright 2017 Edinburgh Genome Foundry, University of Edinburgh). Everyone is welcome to contribute!