SMAG

Soil microbial dark matter mining based on genome-resolved metagenomics

We constructed the SMAG from 3,304 soil metagenomes from large-scale genome-resolved metagenomics to expand the genomic catalog of soil microbiomes!

After download, all datasets can be unpacked using: cat ./mag.tar.gz* > mag.tar.gz; tar -xjvf <mag.tar.gz>

SMAG catalog

Soil MAGs were assembled from 3,304 soil metagenomes from 9 different ecosystems across globe. All the MAGs were recovered for individual metagenomic assemblies using three different tools with default options: Metabat (v2.12.1), MaxBin (v2.2.6), CONCOCT (v1.0.0), which all on the basis of tetranucleotide frequencies(TNF) and coverage information. The resulting MAGs were refined using the module ‘bin_refinement’ from metaWRAP (v1.2.1).

<b>SMAG MAGs (N=40,039)</b>
The SMAG catalog of the soil metagenomes, SNV catalogs and viruses predicted from SMAG for this publication are available at Zenodo. To upload the large gz file, we split it into smaller file with the prefix "mag.tar.gz", downloaders can use the cat ./mag.tar.gz* > mag.tar.gz; tar -xjvf <mag.tar.gz> to process the MAGs.

<b>SMAG MAGs after dereplication (N=21,077)</b> All the 21,077 MAGs were deposited at [cyverse] (https://data.cyverse.org/dav-anon/iplant/home/lucyzju/Caiyu_SMAG_catalog_2023/MAGdrep.tar.gz), All the 40,039 MAGs were deposited at [cyverse] (https://data.cyverse.org/dav-anon/iplant/home/lucyzju/Caiyu_SMAG_catalog_2023/MAG.tar.gz)

• All MAGs are estimated to be >= 50% complete and < 10% contaminated
• The MAGs after dereplication meet or exceed the medium-quality level of the minimum information about a metagenome-assembled genome (MIMAG).
• If you need more information please contact lucy20@zju.edu.cn.