Olink Data Analysis

Lunch seminar Center for Molecular Medicine November 7th.

Table of contents

• Olink Data Analysis
  • Table of contents
  • R and Rstudio basics
  • Dataset
  • Learning objectives
  • Other resources

R and Rstudio basics

If you are not familiar with R and Rstudio, we recomend going over the following tutorials before the seminar:

1. Getting started with R and Rstudio
2. Basic basics
3. Clean it up

Dataset

A synthetic dataset with 500 individuals has been artificially generated using as reference a human dataset consisting of 92 human protein biomarkers from the Proseek Multiplex CVD 1 Olink panel. The toy dataset can be found in the docs/ folder in this repository.

Learning objectives

In this workshop we will use the dataset to:

1. Compare OLINK protein profiles between cases and controls, as well as between the disease sub-groups: Disease subgroups have been adjusted to mimic those of Diaz-Gallo et. al. 2022.
2. Investigate whether there is a significant different between protein profiles between males and females
3. Investigate if differences between protein profiles across subgroups may be influced by treatment of two commonly used drugs to treat SLE

Other resources

Olink-analyze: Olink R package.
Stat Analyzer: Interactive tool to carry out statistical analysis. You must first create an account, login and go to the tools and resources section.
Cheat sheet: Olink analysis cheat sheet.